GBX2 Double Nickase Plasmid (h): sc-404749-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GBX2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GBX2 Double Nickase Plasmid (h) and GBX2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GBX2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GBX2 Antibody (BB-9): sc-81963
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GBX2 Double Nickase Plasmid (h) | sc-404749-NIC | 20 µg | $410.00 | |||
GBX2 Double Nickase Plasmid (h2) | sc-404749-NIC-2 | 20 µg | $410.00 |
GBX2 (gastrulation brain homeobox 2) encodes a homeobox transcription factor that helps specify anterior–posterior patterning and neural plate/brain regionalization during embryonic development. By binding regulatory DNA elements and coordinating transcriptional programs, GBX2 interfaces with developmental signaling networks that include WNT, FGF, and retinoic acid pathways, shaping lineage decisions and tissue identity. Dysregulated GBX2 expression has been associated with altered differentiation states and proliferation programs reported in several tumor contexts, making it relevant for studies of developmental gene regulatory circuits and oncogenic rewiring.
GBX2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GBX2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GBX2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GBX2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GBX2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.