Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GBP5 CRISPR/Cas9 KO Plasmid (h): sc-403674
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GBP5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GBP5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GBP5 CRISPR/Cas9 KO Plasmid (h) | sc-403674 | 20 µg | $397.00 |
Overview
Guanylate-binding protein 5 (GBP5) is an interferon-inducible large GTPase that functions as an antimicrobial and immunoregulatory effector in innate immune cells. It participates in interferon signaling programs and has been linked to inflammasome regulation, including modulation of IL-1 family cytokine output during pathogen sensing. GBP5 contributes to host defense responses against intracellular microbes and shapes inflammatory signaling networks that intersect with NF-κB and cytokine-driven transcriptional states. Dysregulated GBP5 expression has been associated with inflammatory pathologies and immune-related disease signatures, making it relevant for studies of myeloid activation, infection biology, and immunometabolism.
GBP5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GBP5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GBP5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GBP5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GBP5 protein expression.
This CRISPR knockout system enables efficient generation of GBP5-deficient cell models for investigation of GBP5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GBP5 exon(s) critical for GBP5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GBP5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GBP5 CRISPR/Cas9 KO Plasmid (h) and GBP5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GBP5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GBP5 HDR Plasmid (h) and GBP5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GBP5 homology arms to support homology-directed repair at defined GBP5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.