Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GBP4 CRISPR/Cas9 KO Plasmid (h): sc-405870
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GBP4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GBP4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GBP4 CRISPR/Cas9 KO Plasmid (h) | sc-405870 | 20 µg | $397.00 |
Overview
Guanylate binding protein 4 (GBP4) is an interferon-inducible large GTPase that participates in cell-intrinsic immunity downstream of type I and type II interferon signaling. GBP4 is implicated in innate immune effector programs that modulate pathogen restriction, inflammatory signaling, and antiviral responses, with functional ties to JAK–STAT–driven transcriptional networks and interferon-stimulated gene pathways. By influencing immune cell activation states and cytokine-regulated processes, GBP4 expression and activity are frequently examined in contexts of infection biology and immune-associated pathophysiology. Its role in coordinating GTPase-dependent responses makes GBP4 a useful node for studying stimulus-dependent remodeling of cellular defense programs.
GBP4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GBP4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GBP4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GBP4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GBP4 protein expression.
This CRISPR knockout system enables efficient generation of GBP4-deficient cell models for investigation of GBP4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GBP4 exon(s) critical for GBP4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GBP4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GBP4 CRISPR/Cas9 KO Plasmid (h) and GBP4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GBP4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GBP4 HDR Plasmid (h) and GBP4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GBP4 homology arms to support homology-directed repair at defined GBP4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.