Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GBP2 Double Nickase Plasmid (h): sc-402140-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GBP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GBP2 Double Nickase Plasmid (h) and GBP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GBP2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GBP2 Antibody (G-9): sc-271568
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GBP2 Double Nickase Plasmid (h) | sc-402140-NIC | 20 µg | $410.00 | |||
GBP2 Double Nickase Plasmid (h2) | sc-402140-NIC-2 | 20 µg | $410.00 |
Human GBP2 encodes guanylate-binding protein 2, an interferon-inducible large GTPase that functions as an effector of innate immunity. GBP2 is upregulated downstream of IFN-γ/STAT1 signaling and contributes to cell-intrinsic antimicrobial responses, including pathogen restriction at intracellular membranes and coordination of inflammasome-associated processes. Through interactions that influence cytoskeletal dynamics and vesicular trafficking, GBP2 can modulate immune signaling outputs and cellular stress responses. Dysregulated GBP2 expression has been linked to inflammatory pathologies and immune-oncology contexts, making it a useful target for mechanistic studies of interferon-driven programs.
GBP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GBP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GBP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GBP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GBP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.