Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GBP1 Double Nickase Plasmid (h): sc-401778-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GBP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GBP1 Double Nickase Plasmid (h) and GBP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GBP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GBP1 Antibody (1B1): sc-53857
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GBP1 Double Nickase Plasmid (h) | sc-401778-NIC | 20 µg | $410.00 | |||
GBP1 Double Nickase Plasmid (h2) | sc-401778-NIC-2 | 20 µg | $410.00 |
Human GBP1 (guanylate binding protein 1) is an interferon-inducible large GTPase that localizes to intracellular membranes and pathogen-containing vacuoles, where it supports cell-autonomous immunity. GBP1 participates in IFN-γ–driven programs, innate immune signaling, and antimicrobial restriction through GTP-dependent oligomerization and recruitment of downstream effector mechanisms. Its activity intersects with pathways controlling inflammasome regulation, cytokine responses, and epithelial barrier defense, making GBP1 a useful node for studying host–pathogen interactions and inflammatory network wiring. Dysregulated interferon/GBP1 signatures have been reported across multiple inflammatory and infectious disease contexts and are frequently observed in tumor microenvironments, supporting research into immune-mediated stress responses and cancer-associated inflammation.
GBP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GBP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GBP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GBP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GBP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.