GBP1 Antibody (1B1): sc-53857

GBP1 Antibody (1B1) is a rat monoclonal IgG1 antibody that detects GBP1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-GBP1 antibody (1B1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GBP1, or guanylate binding protein 1, is a 592 amino acid protein that belongs to the GTPase protein family and is crucial for binding guanine nucleotides such as GMP, GDP, and GTP. GBP1′s ability to hydrolyze GMP to GTP through two consecutive cleavage steps underscores its importance in cellular signaling pathways. GBP1 is primarily localized in the cytoplasm and is expressed in endothelial cells of the vascular system, where interferon-gamma induces expression during macrophage activation. GBP1 plays a significant role in regulating matrix metalloproteinase-1 expression, which is involved in endothelial cell proliferation and invasiveness. GBP1 is integral to inflammatory cytokine regulation and provides protection against vesicular stomatitis and encephalomyocarditis viruses. GBP1 expression is markedly elevated in vessels of skin diseases such as psoriasis and Kaposi′s sarcoma, establishing GBP1 as a novel cellular activation marker that characterizes inflammatory responses in endothelial cells.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GBP1 Antibody (1B1) References:

1. Interferon-induced guanylate binding protein-1 (GBP-1) mediates an antiviral effect against vesicular stomatitis virus and encephalomyocarditis virus.  |  Anderson, SL., et al. 1999. Virology. 256: 8-14. PMID: 10087221
2. Genomic organization and chromosomal localization of a new member of the murine interferon-induced guanylate-binding protein family.  |  Anderson, SL., et al. 1999. J Interferon Cytokine Res. 19: 487-94. PMID: 10386861
3. Nucleotide-binding characteristics of human guanylate-binding protein 1 (hGBP1) and identification of the third GTP-binding motif.  |  Praefcke, GJ., et al. 1999. J Mol Biol. 292: 321-32. PMID: 10493878
4. Structure of human guanylate-binding protein 1 representing a unique class of GTP-binding proteins.  |  Prakash, B., et al. 2000. Nature. 403: 567-71. PMID: 10676968
5. The helical domain of GBP-1 mediates the inhibition of endothelial cell proliferation by inflammatory cytokines.  |  Guenzi, E., et al. 2001. EMBO J. 20: 5568-77. PMID: 11598000
6. Guanylate-binding protein-1 expression is selectively induced by inflammatory cytokines and is an activation marker of endothelial cells during inflammatory diseases.  |  Lubeseder-Martellato, C., et al. 2002. Am J Pathol. 161: 1749-59. PMID: 12414522
7. The guanylate binding protein-1 GTPase controls the invasive and angiogenic capability of endothelial cells through inhibition of MMP-1 expression.  |  Guenzi, E., et al. 2003. EMBO J. 22: 3772-82. PMID: 12881412
8. How guanylate-binding proteins achieve assembly-stimulated processive cleavage of GTP to GMP.  |  Ghosh, A., et al. 2006. Nature. 440: 101-4. PMID: 16511497
9. Transient kinetic investigation of GTP hydrolysis catalyzed by interferon-gamma-induced hGBP1 (human guanylate binding protein 1).  |  Kunzelmann, S., et al. 2006. J Biol Chem. 281: 28627-35. PMID: 16873363
10. Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis.  |  Fisch, D., et al. 2019. EMBO J. 38: e100926. PMID: 31268602
11. PIM1 controls GBP1 activity to limit self-damage and to guard against pathogen infection.  |  Fisch, D., et al. 2023. Science. 382: eadg2253. PMID: 37797010
12. Native architecture of a human GBP1 defense complex for cell-autonomous immunity to infection.  |  Zhu, S., et al. 2024. Science. 383: eabm9903. PMID: 38422126

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GBP1 Antibody (1B1) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-GBP1 Antibody (1B1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GBP1 Antibody (1B1) Specifications

Alternate Product Name: GBP1

Product Clone Name: 1B1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: GBP1

Epitope Species: human

Product Reactivity/Detection: GBP1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 24 citations in various scientific publications

Available Conjugates/Forms for GBP1 Antibody (1B1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred GBP1-5 antibody is GBP1-5 Antibody (G-12): sc-166960.