GBDR1 CRISPR/Cas9 KO Plasmid (h): sc-409781
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GBDR1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GBDR1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GBDR1 CRISPR/Cas9 KO Plasmid (h) | sc-409781 | 20 µg | $397.00 |
Overview
UBAC1 (also reported as GBDR1) encodes a ubiquitin-associated domain–containing protein implicated in ubiquitin-dependent protein quality control and regulation of protein turnover. By engaging ubiquitinated substrates and interfacing with the ubiquitin–proteasome system, UBAC1 is positioned to influence pathways governing proteostasis, stress responses, and signaling dynamics through controlled degradation of pathway components. Dysregulation of ubiquitination networks is broadly linked to aberrant inflammatory signaling, altered cell-cycle control, and neurodegenerative and oncogenic processes, making UBAC1/GBDR1 a relevant node for mechanistic studies of ubiquitin-mediated regulation. Investigating UBAC1 function can help clarify how ubiquitin recognition and trafficking contribute to pathway robustness under basal and stress conditions.
GBDR1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the UBAC1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the UBAC1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the UBAC1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GBDR1 protein expression.
This CRISPR knockout system enables efficient generation of UBAC1-deficient cell models for investigation of GBDR1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting UBAC1 exon(s) critical for GBDR1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple UBAC1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GBDR1 CRISPR/Cas9 KO Plasmid (h) and GBDR1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the UBAC1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GBDR1 HDR Plasmid (h) and GBDR1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by UBAC1 homology arms to support homology-directed repair at defined UBAC1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.