Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GATA-6 Antibody (F-3): sc-518050. Western blot analysis of GATA-6 expression in HeLa (A), PC-3 (B) and MCF7 (C) nuclear extracts. Detection reagent used: m-IgGκ BP-HRP (Cruz Marker): sc-516102-CM.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GATA-6 CRISPR Activation Plasmid (h): sc-400724-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GATA-6 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- GATA-6 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by GATA-6 CRISPR Activation Plasmid (h) and GATA-6 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the GATA6 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GATA-6 Antibody (3H3-H6-H11): sc-517554
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GATA-6 CRISPR Activation Plasmid (h) | sc-400724-ACT | 20 µg | $397.00 |