gasdermin C CRISPR/Cas9 KO Plasmid (m): sc-429915
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- gasdermin C CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the gasdermin C genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
gasdermin C CRISPR/Cas9 KO Plasmid (m) | sc-429915 | 20 µg | $397.00 |
Overview
Mouse Gsdmc encodes gasdermin C, a member of the gasdermin family implicated in regulated cell death and inflammatory membrane permeabilization downstream of protease activation. Gasdermins can act as executioners of pyroptosis-like processes by forming pores in the plasma membrane, thereby influencing cytokine release, epithelial barrier integrity, and innate immune signaling. Gsdmc expression is enriched in epithelial contexts and has been linked to stress responses that intersect with NF-κB-driven inflammation and tissue remodeling programs. Dysregulated gasdermin activity is therefore relevant to studying inflammatory pathology, host–microbe interactions, and cancer-associated cell death phenotypes in murine models.
gasdermin C CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Gsdmc gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Gsdmc together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Gsdmc open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish gasdermin C protein expression.
This CRISPR knockout system enables efficient generation of Gsdmc-deficient cell models for investigation of gasdermin C signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Gsdmc exon(s) critical for gasdermin C function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Gsdmc genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by gasdermin C CRISPR/Cas9 KO Plasmid (m) and gasdermin C CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Gsdmc locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by gasdermin C HDR Plasmid (m) and gasdermin C HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Gsdmc homology arms to support homology-directed repair at defined Gsdmc target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.