GAS41 Double Nickase Plasmid (h): sc-404113-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GAS41 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GAS41 Double Nickase Plasmid (h) and GAS41 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting YEATS4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GAS41 Antibody (C-10): sc-393708
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GAS41 Double Nickase Plasmid (h) | sc-404113-NIC | 20 µg | $410.00 | |||
GAS41 Double Nickase Plasmid (h2) | sc-404113-NIC-2 | 20 µg | $410.00 |
YEATS4 encodes GAS41, a nuclear YEATS domain–containing protein that functions as a chromatin-associated regulator of transcription and genome organization. GAS41 participates in chromatin remodeling and histone acetylation–linked processes through interactions with complexes such as SRCAP/TIP60, supporting nucleosome dynamics, transcriptional control, and DNA damage–associated chromatin responses. By influencing cell-cycle progression and gene expression programs, YEATS4/GAS41 has been connected to proliferative phenotypes and genomic copy-number changes reported in several tumor types. These features make YEATS4 a useful target for studying epigenetic regulation, chromatin-dependent signaling, and cancer-relevant transcriptional networks in human cells.
GAS41 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the YEATS4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within YEATS4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt YEATS4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of YEATS4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.