GAS41 CRISPR Activation Plasmid (h): sc-404113-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GAS41 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- GAS41 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by GAS41 CRISPR Activation Plasmid (h) and GAS41 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the YEATS4 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GAS41 Antibody (C-10): sc-393708
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GAS41 CRISPR Activation Plasmid (h) | sc-404113-ACT | 20 µg | $397.00 |
YEATS4 encodes GAS41, a YEATS domain–containing nuclear protein that functions as a chromatin-associated regulator of transcription. GAS41 participates in chromatin remodeling and histone acetylation–linked signaling, including interactions with multiprotein complexes implicated in RNA polymerase II–dependent gene expression and cell-cycle control. Through these epigenetic mechanisms, YEATS4 influences proliferation, genome regulation, and cellular stress responses. Dysregulated YEATS4/GAS41 activity has been reported in cancer-associated contexts, supporting its study in oncogenic transcriptional programs and chromatin-driven disease biology.
GAS41 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous YEATS4 expression without altering the underlying DNA sequence.
GAS41 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the YEATS4 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the YEATS4 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous GAS41 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native YEATS4 locus and enabling the study of GAS41-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of GAS41 pathway restoration in tumor cells with silenced or reduced YEATS4 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.