Gas2 Antibody (F-12): sc-398669
- Gas2 Antibody (F-12) is a mouse monoclonal IgG1 κ Gas2 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 273-298 near the C-terminus of Gas2 of human origin
- Gas2 Antibody (F-12) is recommended for detection of Gas2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Gas2 Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for Gas2 Antibody (F-12) for WB applications. This reagent is now offered in a bundle with Gas2 Antibody (F-12) (see ordering information below).
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Gas2 Antibody (F-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Gas2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Gas2 (F-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gas2, consisting of 313 amino acids and encoded by the human gene GAS2, plays a crucial role in apoptosis by serving as a substrate for caspases, particularly caspase-3 and caspase-7, which cleave Gas2 at Asparagine 278 during programmed cell death. This cleavage leads to the rearrangement of the actin cytoskeleton, resulting in dramatic morphological changes in affected cells, which is essential for proper execution of apoptosis. Gas2 is involved in membrane ruffling, a process critical for cell movement and shape changes. During the G0-G1 transition phase, Gas2 can be phosphorylated on serine residues, influencing localization and function. As a cytoskeleton and peripheral membrane protein, Gas2 co-localizes with actin fibers at the cell border and along stress fibers in growth-arrested fibroblasts, and while mainly membrane-associated, hyperphosphorylation leads to accumulation at membrane ruffles. Gas2 is specifically expressed during growth arrest and found ubiquitously, with highest expression levels in liver, lung, and kidney, although absent in spleen.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Gas2 Antibody (F-12) References:
- Caspase-3 and caspase-7 but not caspase-6 cleave Gas2 in vitro: implications for microfilament reorganization during apoptosis. | Sgorbissa, A., et al. 1999. J Cell Sci. 112 (Pt 23): 4475-82. PMID: 10564664
- The death substrate Gas2 binds m-calpain and increases susceptibility to p53-dependent apoptosis. | Benetti, R., et al. 2001. EMBO J. 20: 2702-14. PMID: 11387205
- Protein products of human Gas2-related genes on chromosomes 17 and 22 (hGAR17 and hGAR22) associate with both microfilaments and microtubules. | Goriounov, D., et al. 2003. J Cell Sci. 116: 1045-58. PMID: 12584248
- Prolactin signals via Stat5 and Oct-1 to the proximal cyclin D1 promoter. | Brockman, JL. and Schuler, LA. 2005. Mol Cell Endocrinol. 239: 45-53. PMID: 15885880
- GAS2 and GAS4, a pair of developmentally regulated genes required for spore wall assembly in Saccharomyces cerevisiae. | Ragni, E., et al. 2007. Eukaryot Cell. 6: 302-16. PMID: 17189486
- The Gas family of proteins of Saccharomyces cerevisiae: characterization and evolutionary analysis. | Ragni, E., et al. 2007. Yeast. 24: 297-308. PMID: 17397106
- Effects of nutrient deprivation and differentiation on the expression of growth-arrest genes (gas and gadd) in F9 embryonal carcinoma cells. | Fleming, JV., et al. 1998. Biochem J. 330 (Pt 1): 573-9. PMID: 9461558
- cDNA characterization and chromosome mapping of the human GAS2 gene. | Collavin, L., et al. 1998. Genomics. 48: 265-9. PMID: 9521882
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gas2 Antibody (F-12) | sc-398669 | 200 µg/ml | $316.00 | |||
Gas2 Antibody (F-12): m-IgG1 BP-HRP Bundle | sc-531768 | 200 µg Ab; 20 µg BP | $354.00 | |||
Gas2 Antibody (F-12) AC | sc-398669 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Gas2 Antibody (F-12) HRP | sc-398669 HRP | 200 µg/ml | $316.00 | |||
Gas2 Antibody (F-12) FITC | sc-398669 FITC | 200 µg/ml | $330.00 | |||
Gas2 Antibody (F-12) PE | sc-398669 PE | 200 µg/ml | $343.00 | |||
Gas2 Antibody (F-12) Alexa Fluor® 488 | sc-398669 AF488 | 200 µg/ml | $357.00 | |||
Gas2 Antibody (F-12) Alexa Fluor® 546 | sc-398669 AF546 | 200 µg/ml | $357.00 | |||
Gas2 Antibody (F-12) Alexa Fluor® 594 | sc-398669 AF594 | 200 µg/ml | $357.00 | |||
Gas2 Antibody (F-12) Alexa Fluor® 647 | sc-398669 AF647 | 200 µg/ml | $357.00 | |||
Gas2 Antibody (F-12) Alexa Fluor® 680 | sc-398669 AF680 | 200 µg/ml | $357.00 | |||
Gas2 Antibody (F-12) Alexa Fluor® 790 | sc-398669 AF790 | 200 µg/ml | $357.00 | |||
Gas2 (F-12) Neutralizing Peptide | sc-398669 P | 100 µg/0.5 ml | $68.00 |