GAPDH Antibody (H-12): sc-166574
- GAPDH Antibody (H-12) is a mouse monoclonal IgG2a κ, cited in 277 publications, provided at 200 µg/ml
- raised against a peptide mapping within an internal region of GAPDH of human origin
- GAPDH Antibody (H-12) is recommended for detection of GAPDH of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
- Anti-GAPDH Antibody (H-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GAPDH (H-12): sc-166574.
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GAPDH Antibody (H-12) for WB and IHC(P) applications. These reagents are now offered in bundles with GAPDH Antibody (H-12) (see ordering information below).
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SEE ALSO...
GAPDH Antibody (H-12) is a mouse monoclonal IgG2a kappa light chain antibody raised against a peptide mapping within an internal region of human GAPDH. Anti-GAPDH antibody (H-12) shows reactivity in mouse, rat, and human samples, making it suitable for use in various applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is primarily known for its key role in glycolysis, where GAPDH catalyzes the oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of NAD+ and inorganic phosphate. GAPDH functions as a multifunctional protein involved in several critical cellular processes beyond metabolism. During apoptosis, GAPDH translocates to the nucleus, influencing nuclear events such as transcription activation, DNA replication, and repair. Nuclear localization of GAPDH reveals involvement in processes like RNA binding, nuclear RNA export, and DNA repair, emphasizing importance in maintaining cellular integrity. Through interactions with neuronal proteins associated with neurodegenerative diseases, including β-amyloid precursor and Huntingtin, GAPDH plays a role in neuronal disorders. GAPDH overexpression has been linked to various cancers, such as liver, lung, and prostate cancers, making GAPDH a significant marker in both cancer and neurodegenerative research. GAPDH (H-12) antibody serves as a versatile tool for exploring diverse roles in cellular metabolism, apoptosis, cancer biology, and neurodegenerative diseases across multiple experimental platforms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GAPDH Antibody (H-12) | sc-166574 | 200 µg/ml | $316.00 | |||
GAPDH Antibody (H-12): m-IgGκ BP-HRP Bundle | sc-521661 | 200 µg Ab, 40 µg BP | $354.00 | |||
GAPDH Antibody (H-12): m-IgG2a BP-HRP Bundle | sc-546102 | 200 µg Ab; 10 µg BP | $354.00 | |||
GAPDH Antibody (H-12) AC | sc-166574 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GAPDH Antibody (H-12) HRP | sc-166574 HRP | 200 µg/ml | $316.00 | |||
GAPDH Antibody (H-12) FITC | sc-166574 FITC | 200 µg/ml | $330.00 | |||
GAPDH Antibody (H-12) PE | sc-166574 PE | 200 µg/ml | $343.00 | |||
GAPDH Antibody (H-12) Alexa Fluor® 488 | sc-166574 AF488 | 200 µg/ml | $357.00 | |||
GAPDH Antibody (H-12) Alexa Fluor® 546 | sc-166574 AF546 | 200 µg/ml | $357.00 | |||
GAPDH Antibody (H-12) Alexa Fluor® 594 | sc-166574 AF594 | 200 µg/ml | $357.00 | |||
GAPDH Antibody (H-12) Alexa Fluor® 647 | sc-166574 AF647 | 200 µg/ml | $357.00 | |||
GAPDH Antibody (H-12) Alexa Fluor® 680 | sc-166574 AF680 | 200 µg/ml | $357.00 | |||
GAPDH Antibody (H-12) Alexa Fluor® 790 | sc-166574 AF790 | 200 µg/ml | $357.00 | |||
GAPDH (H-12) Neutralizing Peptide | sc-166574 P | 100 µg/0.5 ml | $68.00 |