gankyrin Antibody (3A6C2): sc-101498

gankyrin Antibody (3A6C2) is a mouse monoclonal IgG2b kappa light chain antibody that detects gankyrin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). anti-gankyrin antibody (3A6C2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. gankyrin, a crucial protein associated with hepatocellular carcinoma, plays a significant role in regulating protein-protein interactions that are vital for cell cycle control and protein degradation. gankyrin′s structure is characterized by six ankyrin repeat domains, which facilitate interactions with key regulatory proteins such as retinoblastoma protein (Rb), cyclin-dependent kinase 4 (Cdk4), the 26S proteasome, and melanoma-associated antigen A4 (MAGE-A4). gankyrin interaction with MAGE-A4 is particularly noteworthy, as research shows suppression of anchorage-independent growth in cells that overexpress gankyrin, highlighting potential as a target for immunotherapy in hepatocellular carcinoma. gankyrin′s influence on cell cycle progression in normal hepatocytes further underscores importance in both cancer biology and cellular regulation.

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gankyrin Antibody (3A6C2) References:

1. MAGE-A4 interacts with the liver oncoprotein gankyrin and suppresses its tumorigenic activity.  |  Nagao, T., et al. 2003. J Biol Chem. 278: 10668-74. PMID: 12525503
2. Role of a novel oncogenic protein, gankyrin, in hepatocyte proliferation.  |  Iwai, A., et al. 2003. J Gastroenterol. 38: 751-8. PMID: 14505129
3. The crystal structure of gankyrin, an oncoprotein found in complexes with cyclin-dependent kinase 4, a 19 S proteasomal ATPase regulator, and the tumor suppressors Rb and p53.  |  Krzywda, S., et al. 2004. J Biol Chem. 279: 1541-5. PMID: 14573599
4. The oncoprotein gankyrin binds to MDM2/HDM2, enhancing ubiquitylation and degradation of p53.  |  Higashitsuji, H., et al. 2005. Cancer Cell. 8: 75-87. PMID: 16023600
5. The oncoprotein gankyrin interacts with RelA and suppresses NF-kappaB activity.  |  Higashitsuji, H., et al. 2007. Biochem Biophys Res Commun. 363: 879-84. PMID: 17904523
6. Oncoprotein p28 GANK binds to RelA and retains NF-kappaB in the cytoplasm through nuclear export.  |  Chen, Y., et al. 2007. Cell Res. 17: 1020-9. PMID: 18040287
7. Association of gankyrin protein expression with early clinical stages and insulin-like growth factor-binding protein 5 expression in human hepatocellular carcinoma.  |  Umemura, A., et al. 2008. Hepatology. 47: 493-502. PMID: 18161051
8. p28GANK knockdown-derived reactive oxygen species induces apoptosis through mitochondrial dysfunction mediated by p38 in HepG2 cells.  |  Wang, X., et al. 2008. Int J Oncol. 33: 743-50. PMID: 18813787
9. Overexpression of a new gene P28GANK confers multidrug resistance of gastric cancer cells.  |  Li, X., et al. 2009. Cancer Invest. 27: 129-39. PMID: 19235584
10. Gankyrin regulates cell signaling network.  |  Wang, X., et al. 2016. Tumour Biol. 37: 5675-82. PMID: 26819208
11. Gankyrin as a potential therapeutic target for cancer.  |  Wang, C. and Cheng, L. 2017. Invest New Drugs. 35: 655-661. PMID: 28527132
12. Gankyrin inhibits ferroptosis through the p53/SLC7A11/GPX4 axis in triple-negative breast cancer cells.  |  Lei, M., et al. 2023. Sci Rep. 13: 21916. PMID: 38081931

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-gankyrin Antibody (3A6C2) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-gankyrin Antibody (3A6C2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

gankyrin Antibody (3A6C2) Specifications

Alternate Product Name: gankyrin

Product Clone Name: 3A6C2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: gankyrin

Epitope Species: human

Product Reactivity/Detection: gankyrin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for gankyrin Antibody (3A6C2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin