γB-crystallin Antibody (D-5): sc-377056
- γB-crystallin Antibody (D-5) is a mouse monoclonal IgG1 κ γB-crystallin antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 71-107 within an internal region of γB-crystallin of human origin
- gamma B-crystallin Antibody (D-5) is recommended for detection of γB-crystallin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-gamma B-crystallin Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for γB-crystallin Antibody (D-5) for WB applications. This reagent is now offered in a bundle with γB-crystallin Antibody (D-5) (see ordering information below).
QUICK LINKS
SEE ALSO...
γB-crystallin Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects γB-crystallin of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-γB-crystallin antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. γB-crystallin, also known as CRYGB or CRYG2, is a crucial member of the γ-crystallin family, which consists of seven closely related proteins that play a vital role in maintaining the transparency and refractive index of the vertebrate eye lens. γB-crystallin is primarily located in the lens fibers of the eye, where γB-crystallin contributes to the lens′s structural integrity and optical properties. A unique two-domain beta fold structure allows γB-crystallin to function effectively as a monomer, ensuring that the lens remains clear and functional. Defects in the gene encoding γB-crystallin can lead to cataract formation, a condition characterized by the clouding of the lens, which can significantly impair vision. The ability of γB-crystallin to maintain lens transparency underscores γB-crystallin′s importance in ocular health, making γB-crystallin monoclonal antibody (D-5) an essential tool for researchers studying lens biology and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
γB-crystallin Antibody (D-5) References:
- Structure of the crystallins. | Slingsby, C. and Clout, NJ. 1999. Eye (Lond). 13 (Pt 3b): 395-402. PMID: 10627816
- Novel mutations in the gamma-crystallin genes cause autosomal dominant congenital cataracts. | Santhiya, ST., et al. 2002. J Med Genet. 39: 352-8. PMID: 12011157
- Homology models of human gamma-crystallins: structural study of the extensive charge network in gamma-crystallins. | Salim, A. and Zaidi, ZH. 2003. Biochem Biophys Res Commun. 300: 624-30. PMID: 12507494
- Two affected siblings with nuclear cataract associated with a novel missense mutation in the CRYGD gene. | Messina-Baas, OM., et al. 2006. Mol Vis. 12: 995-1000. PMID: 16943771
- Trinucleotide repeat polymorphism at the CRYG1 locus. | Hearne, CM. and Todd, JA. 1991. Nucleic Acids Res. 19: 5450. PMID: 1923840
- Human gamma-crystallin genes. A gene family on its way to extinction. | Brakenhoff, RH., et al. 1990. J Mol Biol. 216: 519-32. PMID: 2258929
- Linkage of polymorphic congenital cataract to the gamma-crystallin gene locus on human chromosome 2q33-35. | Rogaev, EI., et al. 1996. Hum Mol Genet. 5: 699-703. PMID: 8733140
- The Cryner element in the murine gamma-crystallin promoters interacts with lens proteins. | Stöger, T., et al. 1997. Ophthalmic Res. 29: 161-71. PMID: 9211469
- The crystallins: genes, proteins and diseases. | Graw, J. 1997. Biol Chem. 378: 1331-48. PMID: 9426193
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
γB-crystallin Antibody (D-5) | sc-377056 | 200 µg/ml | $316.00 | |||
γB-crystallin Antibody (D-5): m-IgG Fc BP-HRP Bundle | sc-526076 | 200 µg Ab; 10 µg BP | $354.00 | |||
γB-crystallin Antibody (D-5) AC | sc-377056 AC | 500 µg/ml, 25% agarose | $416.00 | |||
γB-crystallin Antibody (D-5) HRP | sc-377056 HRP | 200 µg/ml | $316.00 | |||
γB-crystallin Antibody (D-5) FITC | sc-377056 FITC | 200 µg/ml | $330.00 | |||
γB-crystallin Antibody (D-5) PE | sc-377056 PE | 200 µg/ml | $343.00 | |||
γB-crystallin Antibody (D-5) Alexa Fluor® 488 | sc-377056 AF488 | 200 µg/ml | $357.00 | |||
γB-crystallin Antibody (D-5) Alexa Fluor® 546 | sc-377056 AF546 | 200 µg/ml | $357.00 | |||
γB-crystallin Antibody (D-5) Alexa Fluor® 594 | sc-377056 AF594 | 200 µg/ml | $357.00 | |||
γB-crystallin Antibody (D-5) Alexa Fluor® 647 | sc-377056 AF647 | 200 µg/ml | $357.00 | |||
γB-crystallin Antibody (D-5) Alexa Fluor® 680 | sc-377056 AF680 | 200 µg/ml | $357.00 | |||
γB-crystallin Antibody (D-5) Alexa Fluor® 790 | sc-377056 AF790 | 200 µg/ml | $357.00 | |||
γB-crystallin (D-5) Neutralizing Peptide | sc-377056 P | 100 µg/0.5 ml | $68.00 |