G_{α s/olf} Antibody (G-10): sc-365855

Gα s/olf Antibody (G-10) is a mouse monoclonal IgG1 kappa light chain antibody engineered to detect the Gα s and Gα olf subunits of heterotrimeric G proteins, also known as the GNAS-encoded G protein alpha subunit or GNAL Olfactory protein. anti-Gα s/olf antibody (G-10) targets an epitope mapping between amino acids 369-394 at the C-terminus of the rat Gα s protein, ensuring precise and reliable binding. The Gα s and Gα olf proteins undergo essential post-translational modifications, including palmitoylation and phosphorylation, which are critical for their proper localization to the cell membrane and for regulating their signaling activity. These modifications are vital for the interaction of G proteins with cell surface receptors and intracellular effectors, thereby modulating key physiological processes such as hormone signaling and olfactory perception. Gα s/olf (G-10) antibody exhibits broad species reactivity, effectively recognizing Gα s and Gα olf proteins from mouse, rat, and human origins, as well as additional species including canine, bovine, porcine, and avian, making it a versatile tool for cross-species research applications. Gα s/olf (G-10) monoclonal antibody is compatible with a wide range of laboratory techniques, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA), facilitating comprehensive analysis of G protein-mediated pathways. Available in both conjugated and non-conjugated forms, Gα s/olf (G-10) antibody offers flexibility to meet diverse experimental needs.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{α s/olf} Antibody (G-10) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{α s/olf} Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{α s/olf} Antibody (G-10) Specifications

Alternate Product Name: G-protein subunit GNAS locus / GNAL Olfactory

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 369-394

Epitope Target: Gα s

Epitope Species: rat

Product Reactivity/Detection: G_{α s} and G_{α olf}

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for G_{α s/olf} Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred G_{α s} antibody is G_{α s} Antibody (12): sc-135914.