G_{α i-3} Antibody (H-7): sc-365422

Gαi-3 Antibody (H-7) is a mouse monoclonal IgG3 kappa light chain antibody engineered to recognize Gαi-3, also known as GNAI3, from rat origin. Anti-Gαi-3 antibody (H-7) targets an epitope spanning amino acids 339-354 at the C-terminus of Gαi-3, enabling detection in mouse, rat, human, and additional species including equine, canine, bovine, porcine, and avian samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) assays. Gαi-3 is a member of the heterotrimeric G protein family, which plays a crucial role in transducing signals from cell surface receptors to intracellular effectors. Structurally, Gαi-3 consists of a GTP-binding domain and a helical domain, essential for binding and hydrolyzing GTP, which regulates activation and interaction with downstream effectors such as adenylyl cyclase. Post-translational modifications, including myristoylation and phosphorylation, are vital for proper localization and function of Gαi-3, influencing interaction with receptors and other signaling molecules. Gαi-3 interacts with various proteins, including G protein-coupled receptors (GPCRs), beta-gamma subunits, and effectors like ion channels and kinases, facilitating diverse cellular responses such as inhibition of adenylyl cyclase, modulation of ion channel activity, and regulation of cell growth and differentiation. Gαi-3 (H-7) monoclonal antibody is available in both conjugated and non-conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatile options for experimental applications. Gαi-3 (H-7) antibody is an essential tool for researchers studying G protein-mediated signaling pathways, cellular communication, and molecular mechanisms underlying various physiological and pathological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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G_{α i-3} Antibody (H-7) References:

1. Selective tissue distribution of G protein gamma subunits, including a new form of the gamma subunits identified by cDNA cloning.  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
2. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
3. Gustducin is a taste-cell-specific G protein closely related to the transducins.  |  McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
4. Effect of halothane on galphai-3 and its coupling to the M2 muscarinic receptor.  |  Jin, F., et al. 2005. Anesthesiology. 103: 1015-25. PMID: 16249676
5. Diversity of G proteins in signal transduction.  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
6. Biochemical characterization of three stimulatory GTP-binding proteins. The large and small forms of Gs and the olfactory-specific G-protein, Golf.  |  Jones, DT., et al. 1990. J Biol Chem. 265: 2671-6. PMID: 2105931
7. GNAI1 and GNAI3 Reduce Colitis-Associated Tumorigenesis in Mice by Blocking IL6 Signaling and Down-regulating Expression of GNAI2.  |  Li, ZW., et al. 2019. Gastroenterology. 156: 2297-2312. PMID: 30836096
8. Bell-shaped agonist activation of 5-HT1A receptor-coupled Gαi3 G-proteins: Receptor density-dependent switch in receptor signaling.  |  Newman-Tancredi, A., et al. 2019. Cell Signal. 63: 109383. PMID: 31376526
9. Beyond the G protein α subunit: investigating the functional impact of other components of the Gαi3 heterotrimers.  |  Rysiewicz, B., et al. 2023. Cell Commun Signal. 21: 279. PMID: 37817242
10. The polybasic region in Gαi proteins: Relevant or not? Insights from Gαi3 research.  |  Rysiewicz, B., et al. 2024. Cell Signal. 118: 111138. PMID: 38467243
11. Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors.  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779
12. GAIP, a Galphai-3-binding protein, is associated with Golgi-derived vesicles and protein trafficking.  |  Wylie, F., et al. 1999. Am J Physiol. 276: C497-506. PMID: 9950778

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{α i-3} Antibody (H-7) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{α i-3} Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{α i-3} Antibody (H-7) Specifications

Alternate Product Name: G protein subunit GNAI3

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 339-354

Epitope Target: Gα i-3

Epitope Species: rat

Product Reactivity/Detection: G_{α i-1} and G_{α i-3}

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for G_{α i-3} Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred G_{α i-2} antibody is G_{α i-2} Antibody (L5): sc-13534.