Gα i-1/GNAI1 Antibody (B-11): sc-515658

Gα i-1 Antibody (B-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects Gα i-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Gα i-1 antibody (B-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gα i-1 plays a crucial role in cellular signaling as a member of the heterotrimeric G protein family, which transmits signals from cell surface receptors to intracellular effectors. Specifically, Gα i-1 inhibits adenylate cyclase activity, leading to a decrease in cyclic AMP levels, which is vital for regulating various physiological processes such as neurotransmitter release, hormone secretion, and cell growth. Gα i-1′s interaction with specific receptors and downstream effectors allows modulation of diverse signaling pathways, making Gα i-1 an important target for research in areas such as neurobiology and cardiovascular health. Understanding Gα i-1 function and interactions can provide insights into the mechanisms underlying various diseases and potential therapeutic strategies.

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Gα i-1/GNAI1 Antibody (B-11) References:

1. Extracellular α-synuclein induces sphingosine 1-phosphate receptor subtype 1 uncoupled from inhibitory G-protein leaving β-arrestin signal intact.  |  Zhang, L., et al. 2017. Sci Rep. 7: 44248. PMID: 28300069
2. G protein-coupled estrogen receptor 1 (GPER1)/GPR30 increases ERK1/2 activity through PDZ motif-dependent and -independent mechanisms.  |  Gonzalez de Valdivia, E., et al. 2017. J Biol Chem. 292: 9932-9943. PMID: 28450397
3. Extracellular α-synuclein drives sphingosine 1-phosphate receptor subtype 1 out of lipid rafts, leading to impaired inhibitory G-protein signaling.  |  Badawy, SMM., et al. 2018. J Biol Chem. 293: 8208-8216. PMID: 29632069
4. Role of MxB in Alpha Interferon-Mediated Inhibition of HIV-1 Infection.  |  Xu, B., et al. 2018. J Virol. 92: PMID: 29925663
5. Functional activation of Gαq/11 protein via α1 -adrenoceptor in rat cerebral cortical membranes.  |  Odagaki, Y., et al. 2019. Clin Exp Pharmacol Physiol. 46: 567-574. PMID: 30869808
6. Eukaryotic translation elongation factor 1 alpha (eEF1A) inhibits Siniperca chuatsi rhabdovirus (SCRV) infection through two distinct mechanisms.  |  Meng, X-Y., et al. 2023. J Virol. 97: e0122623. PMID: 37861337
7. Guanylate-binding protein 1 inhibits inflammatory factors produced by H5N1 virus through Its GTPase activity.  |  Jiang, Y., et al. 2024. Poult Sci. 103: 103800. PMID: 38743966
8. 1,25-Dihydroxyvitamin D3 attenuates adenylyl cyclase activity in rat thyroid cells: reduction of thyrotropin receptor number and increase in guanine nucleotide-binding protein Gi-2 alpha.  |  Berg, JP., et al. 1994. Endocrinology. 135: 595-602. PMID: 8033808
9. The kappa opioid receptor expressed on the mouse R1.1 thymoma cell line is coupled to adenylyl cyclase through a pertussis toxin-sensitive guanine nucleotide-binding regulatory protein.  |  Lawrence, DM. and Bidlack, JM. 1993. J Pharmacol Exp Ther. 266: 1678-83. PMID: 8103800
10. Wortmannin binds specifically to 1-phosphatidylinositol 3-kinase while inhibiting guanine nucleotide-binding protein-coupled receptor signaling in neutrophil leukocytes.  |  Thelen, M., et al. 1994. Proc Natl Acad Sci U S A. 91: 4960-4. PMID: 8197165

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Gα i-1/GNAI1 Antibody (B-11) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Gα i-1/GNAI1 Antibody (B-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Gα i-1/GNAI1 Antibody (B-11) Specifications

Alternate Product Name: G protein subunit GNAI1

Product Clone Name: B-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 91-116

Epitope Target: G_{α i-1}

Epitope Species: rat

Product Reactivity/Detection: G_{α i-1}

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Gα i-1/GNAI1 Antibody (B-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred G_{α i-1}/GNAI1 antibody is G_{α i-1}/GNAI1 Antibody (R4): sc-13533.