G_{α gust} Antibody (B-3): sc-518163

Gα gust Antibody (B-3) is a mouse monoclonal IgM antibody that detects Gα gust protein from mouse, rat, and human sources through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Gα gust (B-3) antibody comes in non-conjugated form and various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® options. Gα gust protein functions as a key molecular switch in gustatory signaling pathways, transmitting signals from taste receptors to downstream effectors within taste cells. Gα gust protein primarily resides in taste buds, where sweet, bitter, and umami flavors are detected. Gα gust expression in taste cells enables proper gustatory system function by triggering signaling cascades that lead to taste perception. As a member of the Gi subclass of G protein α subunits, Gα gust inhibits adenylate cyclase activity and regulates intracellular cyclic AMP levels. Research on Gα gust localization and function helps scientists understand taste transduction mechanisms and their impact on dietary preferences and nutritional behaviors.

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G_{α gust} Antibody (B-3) References:

1. Comprehensive study on G protein alpha-subunits in taste bud cells, with special reference to the occurrence of Galphai2 as a major Galpha species.  |  Kusakabe, Y., et al. 2000. Chem Senses. 25: 525-31. PMID: 11015324
2. Bitter taste transduced by PLC-beta(2)-dependent rise in IP(3) and alpha-gustducin-dependent fall in cyclic nucleotides.  |  Yan, W., et al. 2001. Am J Physiol Cell Physiol. 280: C742-51. PMID: 11245589
3. Selective tissue distribution of G protein gamma subunits, including a new form of the gamma subunits identified by cDNA cloning.  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
4. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
5. Gustducin is a taste-cell-specific G protein closely related to the transducins.  |  McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
6. Colocalization of the alpha-subunit of gustducin with PYY and GLP-1 in L cells of human colon.  |  Rozengurt, N., et al. 2006. Am J Physiol Gastrointest Liver Physiol. 291: G792-802. PMID: 16728727
7. Diversity of G proteins in signal transduction.  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
8. Expression of α-gustducin in mammalian retinas.  |  Son, MJ., et al. 2011. Neuroreport. 22: 146-50. PMID: 21200350
9. Diverse contributions of Tas1r2/Tas2rs within the rat and mouse soft palate to sweet and bitter neural responses.  |  Tomonari, H., et al. 2014. Neurosci Lett. 569: 63-7. PMID: 24699177
10. Predicted Structure of Fully Activated Tas1R3/1R3' Homodimer Bound to G Protein and Natural Sugars: Structural Insights into G Protein Activation by a Class C Sweet Taste Homodimer with Natural Sugars.  |  Mafi, A., et al. 2021. J Am Chem Soc. 143: 16824-16838. PMID: 34585929
11. Adiponectin Enhances Fatty Acid Signaling in Human Taste Cells by Increasing Surface Expression of CD36.  |  Lin, F., et al. 2023. Int J Mol Sci. 24: PMID: 36982874
12. Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors.  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{α gust} Antibody (B-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{α gust} Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{α gust} Antibody (B-3) Specifications

Alternate Product Name: Gα gust

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 53-74

Epitope Target: G_{α gust}

Epitope Species: human

Product Reactivity/Detection: G_{α gust}

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for G_{α gust} Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin