G_{α 11} Antibody (D-6): sc-390382

Gα 11 Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects the G alpha 11 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Gα 11 (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. G alpha 11 protein plays a crucial role in cellular signaling as a member of the heterotrimeric G protein family, which transmits signals from activated cell surface receptors to intracellular effectors. Specifically, G alpha 11 is involved in the activation of phospholipase C, leading to the production of inositol trisphosphate and diacylglycerol, which are vital for calcium signaling and various cellular responses. This signaling pathway is essential for numerous physiological processes, including smooth muscle contraction, neurotransmitter release, and cell proliferation. G alpha 11 protein is predominantly expressed in the brain and lung, where G alpha 11 contributes to the regulation of diverse functions such as cardiovascular responses and sensory perception. Understanding G alpha 11′s role in these pathways is important for elucidating G alpha 11′s involvement in various diseases, including cancer and cardiovascular disorders.

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G_{α 11} Antibody (D-6) References:

1. Selective tissue distribution of G protein gamma subunits, including a new form of the gamma subunits identified by cDNA cloning.  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
2. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
3. Gustducin is a taste-cell-specific G protein closely related to the transducins.  |  McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
4. Diversity of G proteins in signal transduction.  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
5. G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates.  |  Strathmann, M. and Simon, MI. 1990. Proc Natl Acad Sci U S A. 87: 9113-7. PMID: 2123549
6. Requirement of Gαq/Gα11 Signaling in the Preservation of Mouse Intestinal Epithelial Homeostasis.  |  Watanabe, N., et al. 2016. Cell Mol Gastroenterol Hepatol. 2: 767-782.e6. PMID: 28174748
7. Knockin mouse with mutant Gα11 mimics human inherited hypocalcemia and is rescued by pharmacologic inhibitors.  |  Roszko, KL., et al. 2017. JCI Insight. 2: e91079. PMID: 28194446
8. Gα11 mutation in mice causes hypocalcemia rectifiable by calcilytic therapy.  |  Gorvin, CM., et al. 2017. JCI Insight. 2: e91103. PMID: 28194447
9. The role of Gαq/Gα11 signaling in intestinal epithelial cells.  |  Mashima, H., et al. 2018. Biochem Biophys Rep. 13: 93-98. PMID: 29387814
10. Effects of Oncogenic Gαq and Gα11 Inhibition by FR900359 in Uveal Melanoma.  |  Lapadula, D., et al. 2019. Mol Cancer Res. 17: 963-973. PMID: 30567972
11. Direct targeting of Gαq and Gα11 oncoproteins in cancer cells.  |  Annala, S., et al. 2019. Sci Signal. 12: PMID: 30890659
12. Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors.  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{α 11} Antibody (D-6) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{α 11} Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{α 11} Antibody (D-6) Specifications

Alternate Product Name: G protein subunit GNA11

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 3-39

Epitope Target: Gα 11

Epitope Species: mouse

Product Reactivity/Detection: G_{α 11}

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for G_{α 11} Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred G_{α q/11/14} antibody is G_{α q/11/14} Antibody (G-7): sc-365906.