Gads Antibody (G-11): sc-390373

Gads Antibody (G-11) is a mouse monoclonal IgG1 antibody that detects Gads in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Gads, an essential adapter protein, plays a critical role in cellular signaling by linking tyrosine-phosphorylated proteins, such as Shc, to downstream effectors, thereby facilitating the transmission of signals from activated growth factor receptors. This interaction is vital for the regulation of various cellular processes, including proliferation, differentiation, and survival. The presence of both SH2 and SH3 domains in Gads enables complex protein-protein interactions, which are crucial for maintaining the balance of signaling pathways within the cell. Dysregulation of Gads or associated pathways can lead to aberrant cellular responses, contributing to the development of various diseases, including cancer. anti-Gads antibody (G-11) serves as an invaluable tool for researchers exploring the intricate signaling networks that govern cellular behavior and investigating potential therapeutic targets within these pathways.

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Gads Antibody (G-11) References:

1. Association of Grb2, Gads, and phospholipase C-gamma 1 with phosphorylated LAT tyrosine residues. Effect of LAT tyrosine mutations on T cell angigen receptor-mediated signaling.  |  Zhang, W., et al. 2000. J Biol Chem. 275: 23355-61. PMID: 10811803
2. Caspase-dependent cleavage of the hematopoietic specific adaptor protein Gads alters signalling from the T cell receptor.  |  Berry, DM., et al. 2001. Oncogene. 20: 1203-11. PMID: 11313864
3. Evidence for the requirement of ITAM domains but not SLP-76/Gads interaction for integrin signaling in hematopoietic cells.  |  Abtahian, F., et al. 2006. Mol Cell Biol. 26: 6936-49. PMID: 16943434
4. CD28 and Grb-2, relative to Gads or Grap, preferentially co-operate with Vav1 in the activation of NFAT/AP-1 transcription.  |  Schneider, H. and Rudd, CE. 2008. Biochem Biophys Res Commun. 369: 616-21. PMID: 18295596
5. Differential roles for the adapters Gads and LAT in platelet activation by GPVI and CLEC-2.  |  Hughes, CE., et al. 2008. J Thromb Haemost. 6: 2152-9. PMID: 18826392
6. Gads-deficient thymocytes are blocked at the transitional single positive CD4+ stage.  |  Dalheimer, SL., et al. 2009. Eur J Immunol. 39: 1395-404. PMID: 19337995
7. Quantitative analysis by surface plasmon resonance of CD28 interaction with cytoplasmic adaptor molecules Grb2, Gads and p85 PI3K.  |  Higo, K., et al. 2014. Immunol Invest. 43: 278-91. PMID: 24475931
8. Epigenetic Suppression of GADs Expression is Involved in Temporal Lobe Epilepsy and Pilocarpine-Induced Mice Epilepsy.  |  Wang, JG., et al. 2016. Neurochem Res. 41: 1751-60. PMID: 27220336
9. Survival and Developmental Progression of Unselected Thymocytes in the Absence of the T-Cell Adaptor Gads.  |  Shalah, R., et al. 2025. Eur J Immunol. 55: e202451000. PMID: 39989300
10. Gads is a novel SH2 and SH3 domain-containing adaptor protein that binds to tyrosine-phosphorylated Shc.  |  Liu, SK. and McGlade, CJ. 1998. Oncogene. 17: 3073-82. PMID: 9872323

The preferred Gads antibody is Gads Antibody (UW40): sc-73652.