Anti-GABP-alpha Antibody (8C1B10) recommended for detection of GABP-α of human origin by WB, IP and ELISA

Anti-GABP-α Antibody (8C1B10): sc-134222

Anti-GABP-α Antibody (8C1B10) is rated 4.0 out of 5 by 1.
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Datasheets

    • GABP-α Antibody (8C1B10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a recombinant protein corresponding to amino acids 120-190 of GABP-α of human origin
    • recommended for detection of GABP-α of human origin by WB, IP and ELISA
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GABP-α Antibody (8C1B10). This work is in progress.
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How much sample do you recommend loading on the gel for Western blot detection using GABP-α (8C1B10): sc-134222?

Asked by: Cweed
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from WB: positive results in human GABP WB: positive results in human GABP- transfected 293T whole cell lysates and HeLa nuclear extract. -SCBT QC
Date published: 2014-03-10
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