Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GABAB R1 Double Nickase Plasmid (h): sc-401714-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GABAB R1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GABAB R1 Double Nickase Plasmid (h) and GABAB R1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GABBR1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GABAB R1 Antibody (D-2): sc-166408
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GABAB R1 Double Nickase Plasmid (h) | sc-401714-NIC | 20 µg | $410.00 | |||
GABAB R1 Double Nickase Plasmid (h2) | sc-401714-NIC-2 | 20 µg | $410.00 |
GABBR1 encodes the human GABA\_B receptor subunit 1 (GABA\_B R1), an obligate component of heterodimeric G protein–coupled receptors that mediate slow inhibitory neurotransmission in the central nervous system. Upon activation, GABA\_B signaling couples to G\_i/o proteins to inhibit adenylyl cyclase, modulate cAMP/PKA pathways, regulate calcium and potassium channel activity, and suppress neurotransmitter release at pre- and postsynaptic sites. This pathway influences neuronal excitability, synaptic plasticity, and network oscillations, linking GABBR1 to mechanisms that shape circuit function. Altered GABAergic inhibitory tone and dysregulated GABA\_B receptor signaling have been investigated in the context of neurological and neuropsychiatric phenotypes, supporting the use of GABBR1 perturbation models for mechanistic studies.
GABAB R1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GABBR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GABBR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GABBR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GABBR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.