GABAA Rθ CRISPR/Cas9 KO Plasmid (h): sc-406766
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GABAA Rθ CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GABAA Rθ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GABAA Rθ CRISPR/Cas9 KO Plasmid (h) | sc-406766 | 20 µg | $397.00 |
Overview
GABRQ encodes the θ (theta) subunit of the GABA\(_A\) receptor, a pentameric ligand-gated chloride channel that mediates fast inhibitory neurotransmission in the human central nervous system. By regulating chloride influx and membrane hyperpolarization, GABA\(_A\) receptors shape neuronal excitability, synaptic integration, and network oscillations, and they interface functionally with pathways controlling calcium dynamics, neurotransmitter release, and activity-dependent gene expression. Variation in inhibitory tone involving specific receptor subunits is linked to neurodevelopmental and neuropsychiatric phenotypes and can influence susceptibility to seizure-related and stress-associated circuitry dysfunction. Subunit composition, trafficking, and receptor pharmacology are key determinants of inhibitory synapse function, making GABRQ a useful target for dissecting GABAergic signaling mechanisms.
GABAA Rθ CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GABRQ gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GABRQ together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GABRQ open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GABAA Rθ protein expression.
This CRISPR knockout system enables efficient generation of GABRQ-deficient cell models for investigation of GABAA Rθ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GABRQ exon(s) critical for GABAA Rθ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GABRQ genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GABAA Rθ CRISPR/Cas9 KO Plasmid (h) and GABAA Rθ CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GABRQ locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GABAA Rθ HDR Plasmid (h) and GABAA Rθ HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GABRQ homology arms to support homology-directed repair at defined GABRQ target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.