GABAA Rπ CRISPR/Cas9 KO Plasmid (h): sc-404623
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GABAA Rπ CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GABAA Rπ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GABAA Rπ CRISPR/Cas9 KO Plasmid (h) | sc-404623 | 20 µg | $397.00 |
Overview
GABRP encodes the π subunit of the human GABA\(_A\) receptor, a ligand-gated chloride channel that mediates inhibitory neurotransmission and shapes membrane excitability. Incorporation of the π subunit can modulate receptor assembly, ion conductance, and pharmacological properties, thereby influencing synaptic signaling and downstream neuronal network activity. GABA\(_A\) receptor signaling interfaces with calcium-dependent pathways, neuronal development, and homeostatic regulation of excitation–inhibition balance. Altered expression or regulation of GABRP has been investigated in contexts of neuropsychiatric and neurological disease biology, as well as in studies of epithelial cell signaling where GABAergic components can impact proliferation and differentiation programs.
GABAA Rπ CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GABRP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GABRP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GABRP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GABAA Rπ protein expression.
This CRISPR knockout system enables efficient generation of GABRP-deficient cell models for investigation of GABAA Rπ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GABRP exon(s) critical for GABAA Rπ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GABRP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GABAA Rπ CRISPR/Cas9 KO Plasmid (h) and GABAA Rπ CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GABRP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GABAA Rπ HDR Plasmid (h) and GABAA Rπ HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GABRP homology arms to support homology-directed repair at defined GABRP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.