GABAA Rδ Double Nickase Plasmid (h): sc-401954-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GABAA Rδ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GABAA Rδ Double Nickase Plasmid (h) and GABAA Rδ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GABRD. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GABAA Rδ Antibody (H-4): sc-271231
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GABAA Rδ Double Nickase Plasmid (h) | sc-401954-NIC | 20 µg | $410.00 | |||
GABAA Rδ Double Nickase Plasmid (h2) | sc-401954-NIC-2 | 20 µg | $410.00 |
GABRD encodes the δ subunit of the human GABA_A receptor (GABA_A Rδ), an inhibitory ligand-gated chloride channel that contributes prominently to extrasynaptic receptors mediating tonic inhibition in neurons. By shaping membrane excitability and synaptic integration, δ-containing GABA_A receptors influence network oscillations and neurotransmission balance within GABAergic signaling pathways. GABRD expression and receptor composition are tightly regulated during development and in response to neurosteroids, linking this subunit to activity-dependent plasticity. Altered δ-subunit function or expression has been associated with neurological and neuropsychiatric phenotypes, supporting its relevance for mechanistic studies of inhibitory signaling and circuit dysfunction.
GABAA Rδ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GABRD locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GABRD. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GABRD function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GABRD-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.