Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GABAA Rα2 CRISPR/Cas9 KO Plasmid (h): sc-402715
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GABAA Rα2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GABAA Rα2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GABAA Rα2 CRISPR/Cas9 KO Plasmid (h) | sc-402715 | 20 µg | $397.00 |
Overview
GABRA2 encodes the α2 subunit of the human GABA\(_A\) receptor, a pentameric ligand-gated chloride channel that mediates fast inhibitory neurotransmission in the central nervous system. Receptors containing GABA\(_A\) Rα2 shape synaptic inhibition, neuronal excitability, and network oscillations by coupling GABA binding to chloride flux and membrane hyperpolarization. This subunit contributes to receptor assembly, trafficking, and synaptic localization, integrating with signaling processes that regulate ion homeostasis and activity-dependent plasticity. Genetic and functional perturbations of GABRA2 have been associated with neuropsychiatric and seizure-related phenotypes, supporting its relevance for mechanistic studies of inhibitory circuit dysfunction.
GABAA Rα2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GABRA2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GABRA2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GABRA2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GABAA Rα2 protein expression.
This CRISPR knockout system enables efficient generation of GABRA2-deficient cell models for investigation of GABAA Rα2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GABRA2 exon(s) critical for GABAA Rα2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GABRA2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GABAA Rα2 CRISPR/Cas9 KO Plasmid (h) and GABAA Rα2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GABRA2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GABAA Rα2 HDR Plasmid (h) and GABAA Rα2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GABRA2 homology arms to support homology-directed repair at defined GABRA2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.