GABA_A Rα5 Antibody (A-5): sc-393921

GABAA Rα5 Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects GABAA R alpha 5 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-GABAA Rα5 antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GABAA R alpha 5 protein plays a crucial role in the central nervous system as a subunit of the GABAA receptor, which mediates the inhibitory effects of gamma-aminobutyric acid (GABA). This receptor maintains the balance between neuronal excitation and inhibition, influencing various physiological processes such as anxiety, sleep, and muscle relaxation. GABAA R alpha 5 subunit modulates synaptic transmission and is implicated in several neurological disorders, including epilepsy and anxiety disorders. Understanding GABAA R alpha 5 protein function helps researchers explore potential therapeutic targets for these conditions.

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GABA_A Rα5 Antibody (A-5) References:

1. Molecular heterogeneity of the gamma-aminobutyric acid (GABA) transport system. Cloning of two novel high affinity GABA transporters from rat brain.  |  Borden, LA., et al. 1992. J Biol Chem. 267: 21098-104. PMID: 1400419
2. New GABAA receptor alpha5 subunit gene polymorphism that may confound genotyping.  |  Vaquero, C., et al. 2004. Am J Med Genet B Neuropsychiatr Genet. 129B: 27-8. PMID: 15274034
3. Activated radixin is essential for GABAA receptor alpha5 subunit anchoring at the actin cytoskeleton.  |  Loebrich, S., et al. 2006. EMBO J. 25: 987-99. PMID: 16467845
4. GABA: an excitatory transmitter in early postnatal life.  |  Cherubini, E., et al. 1991. Trends Neurosci. 14: 515-9. PMID: 1726341
5. In vitro and in vivo properties of 3-tert-butyl-7-(5-methylisoxazol-3-yl)-2-(1-methyl-1H-1,2,4-triazol-5-ylmethoxy)-pyrazolo[1,5-d]-[1,2,4]triazine (MRK-016), a GABAA receptor alpha5 subtype-selective inverse agonist.  |  Atack, JR., et al. 2009. J Pharmacol Exp Ther. 331: 470-84. PMID: 19704033
6. Preclinical and clinical pharmacology of the GABAA receptor alpha5 subtype-selective inverse agonist alpha5IA.  |  Atack, JR. 2010. Pharmacol Ther. 125: 11-26. PMID: 19770002
7. Cloning of the human brain GABA transporter.  |  Nelson, H., et al. 1990. FEBS Lett. 269: 181-4. PMID: 2387399
8. Altered inhibitory synapses in de novo GABRA5 and GABRA1 mutations associated with early onset epileptic encephalopathies.  |  Hernandez, CC., et al. 2019. Brain. 142: 1938-1954. PMID: 31056671
9. Targeting of the 67-kDa isoform of glutamic acid decarboxylase to intracellular organelles is mediated by its interaction with the NH2-terminal region of the 65-kDa isoform of glutamic acid decarboxylase.  |  Dirkx, R., et al. 1995. J Biol Chem. 270: 2241-6. PMID: 7836456
10. GABAC receptors in the vertebrate retina.  |  Lukasiewicz, PD. 1996. Mol Neurobiol. 12: 181-94. PMID: 8884747
11. Expression cloning of GABA(B) receptors uncovers similarity to metabotropic glutamate receptors.  |  Kaupmann, K., et al. 1997. Nature. 386: 239-46. PMID: 9069281
12. GABA(A)-receptor subtypes: clinical efficacy and selectivity of benzodiazepine site ligands.  |  Korpi, ER., et al. 1997. Ann Med. 29: 275-82. PMID: 9375983

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GABA_A Rα5 Antibody (A-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-GABA_A Rα5 Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GABA_A Rα5 Antibody (A-5) Specifications

Alternate Product Name: GABRA5

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 356-415

Epitope Target: GABA_A Rα5

Epitope Species: human

Product Reactivity/Detection: GABA_A Rα5

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for GABA_A Rα5 Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred GABA_A Rα1-6 antibody is GABA_A Rα1-6 Antibody (E-8): sc-376282.