G6Pase-α CRISPR/Cas9 KO Plasmid (m): sc-420443
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- G6Pase-α CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the G6Pase-α genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
G6Pase-α CRISPR/Cas9 KO Plasmid (m) | sc-420443 | 20 µg | $397.00 |
Overview
G6pc encodes glucose-6-phosphatase alpha (G6Pase-α), an endoplasmic reticulum membrane enzyme that catalyzes the terminal step of gluconeogenesis and glycogenolysis by hydrolyzing glucose-6-phosphate to free glucose and inorganic phosphate. In mouse liver, kidney, and intestinal epithelium, G6Pase-α supports fasting glucose homeostasis and integrates with pathways controlling glycogen turnover, hepatic glucose output, and ER-localized carbohydrate metabolism. Disruption of G6Pase-α activity is mechanistically linked to glycogen storage and metabolic dysregulation phenotypes, making G6pc a key target for studying glucose handling and hepatometabolic stress responses. G6pc models are also used to investigate transcriptional and hormonal control of gluconeogenic programs and ER membrane enzyme function.
G6Pase-α CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the G6pc gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the G6pc together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the G6pc open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish G6Pase-α protein expression.
This CRISPR knockout system enables efficient generation of G6pc-deficient cell models for investigation of G6Pase-α signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting G6pc exon(s) critical for G6Pase-α function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple G6pc genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by G6Pase-α CRISPR/Cas9 KO Plasmid (m) and G6Pase-α CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the G6pc locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by G6Pase-α HDR Plasmid (m) and G6Pase-α HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by G6pc homology arms to support homology-directed repair at defined G6pc target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.