Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
G3BP1 Double Nickase Plasmid (h): sc-400745-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- G3BP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- G3BP1 Double Nickase Plasmid (h) and G3BP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting G3BP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: G3BP1 Antibody (H-10): sc-365338
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
G3BP1 Double Nickase Plasmid (h) | sc-400745-NIC | 20 µg | $410.00 | |||
G3BP1 Double Nickase Plasmid (h2) | sc-400745-NIC-2 | 20 µg | $410.00 |
G3BP1 (G3BP stress granule assembly factor 1) is an RNA-binding protein that coordinates stress granule nucleation and regulates mRNA stability and translation during cellular stress. It links signaling inputs from pathways such as RAS/MAPK and PI3K/AKT to post-transcriptional gene control through interactions with 40S ribosomal subunits, translation initiation factors, and viral or cellular RNAs. G3BP1 also contributes to innate immune signaling and antiviral responses by shaping RNA sensing and ribonucleoprotein dynamics. Dysregulated G3BP1 activity and stress granule biology have been associated with cancer cell adaptation, neurodegeneration, and viral replication mechanisms, making it a useful target for pathway-dissection studies.
G3BP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the G3BP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within G3BP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt G3BP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of G3BP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.