G-CSF Antibody (3D1): sc-53292

G-CSF Antibody (3D1) is a mouse monoclonal IgG2a kappa light chain antibody that detects G-CSF protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-G-CSF antibody (3D1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Granulocyte-colony stimulating factor (G-CSF) is a crucial pleiotropic cytokine that plays a significant role in the differentiation, proliferation, and activation of neutrophilic granulocytes, which are essential for the immune response. G-CSF is primarily secreted by bone marrow stromal cells and acts on hematopoietic progenitor cells to stimulate the production of neutrophils, thereby enhancing the body′s ability to fight infections. The mature G-CSF protein consists of 178 amino acids, following the cleavage of a 30 amino acid signal peptide, and interacts with G-CSF receptor (G-CSFR), a heavily glycosylated polypeptide that spans the cell membrane. This interaction triggers a cascade of intracellular signaling pathways, including the Ras/MAPK pathway, which is vital for cell survival and proliferation. G-CSF has been shown to influence the activation of various kinases and transcription factors, such as Lyn, Syk, and Stat3, underscoring its importance in immune regulation and response.

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G-CSF Antibody (3D1) References:

1. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.  |  Abrams, JS., et al. 1992. Immunol Rev. 127: 5-24. PMID: 1387110
2. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies.  |  Abrams, JS. 2001. Curr Protoc Immunol. Chapter 6: 6.20.1-6.20.15. PMID: 18432811
3. G-CSF and GM-CSF in Neutropenia.  |  Mehta, HM., et al. 2015. J Immunol. 195: 1341-9. PMID: 26254266
4. G-CSF, the guardian of granulopoiesis.  |  Mehta, HM. and Corey, SJ. 2021. Semin Immunol. 54: 101515. PMID: 34772606
5. Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor.  |  Nagata, S., et al. Nature. 319: 415-8. PMID: 3484805
6. Isolation and characterization of the cDNA for murine granulocyte colony-stimulating factor.  |  Tsuchiya, M., et al. 1986. Proc Natl Acad Sci U S A. 83: 7633-7. PMID: 3489940
7. G-CSF drives autoinflammation in APLAID.  |  Mulazzani, E., et al. 2023. Nat Immunol. 24: 814-826. PMID: 36997670
8. G-CSF in the biology and treatment of acute myeloid leukemias.  |  Visani, G. and Manfroi, S. 1995. Leuk Lymphoma. 18: 423-8. PMID: 8528048
9. Granulocyte colony-stimulating factor rapidly activates a distinct STAT-like protein in normal myeloid cells.  |  Tweardy, DJ., et al. 1995. Blood. 86: 4409-16. PMID: 8541528
10. Specific involvement of tyrosine 764 of human granulocyte colony-stimulating factor receptor in signal transduction mediated by p145/Shc/GRB2 or p90/GRB2 complexes.  |  de Koning, JP., et al. 1996. Blood. 87: 132-40. PMID: 8547634
11. CD14+ cells in granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood mononuclear cells induce secretion of interleukin-6 and G-CSF by marrow stroma.  |  Mielcarek, M., et al. 1996. Blood. 87: 574-80. PMID: 8555479
12. Suppression of apoptosis during cytokine deprivation of 32D cells is not sufficient to induce complete granulocytic differentiation.  |  Rodel, JE. and Link, DC. 1996. Blood. 87: 858-64. PMID: 8562953

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G-CSF Antibody (3D1) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-G-CSF Antibody (3D1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G-CSF Antibody (3D1) Specifications

Alternate Product Name: CSF3

Product Clone Name: 3D1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: G-CSF

Epitope Species: human

Product Reactivity/Detection: G-CSF

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for G-CSF Antibody (3D1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, L: Azide-Free, PE: Phycoerythrin