Fyn Double Nickase Plasmid (h): sc-400152-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Fyn Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Fyn Double Nickase Plasmid (h) and Fyn Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FYN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Fyn Antibody (15): sc-434
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fyn Double Nickase Plasmid (h) | sc-400152-NIC | 20 µg | $410.00 | |||
Fyn Double Nickase Plasmid (h2) | sc-400152-NIC-2 | 20 µg | $410.00 |
FYN encodes Fyn, a Src family non-receptor tyrosine kinase that integrates signals from immune receptors, integrins, and growth factor receptors to regulate phosphorylation-driven control of proliferation, survival, cytoskeletal remodeling, and vesicular trafficking. Fyn participates in T cell receptor signaling, B cell receptor pathways, and downstream cascades including PI3K–AKT, MAPK/ERK, and focal adhesion signaling, shaping adhesion and migration responses. In the nervous system, Fyn modulates synaptic plasticity through NMDA receptor and myelin-associated signaling, influencing neuronal differentiation and connectivity. Dysregulated FYN activity or expression is linked to altered immune signaling and oncogenic kinase networks, and has been implicated in neurodegeneration-associated signaling and aberrant cell motility.
Fyn Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FYN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FYN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FYN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FYN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.