Fyb Antibody (C-8): sc-515372

Fyb Antibody (C-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Fyb protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Fyb antibody (C-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Fyb protein, also known as Fyn binding protein, plays a crucial role in T cell signaling by interacting with tyrosine kinase p59fyn and anchoring proteins such as SKAP55 and SKAP55-R. These interactions are vital for regulating signaling events initiated by lymphocyte antigen receptors, which ultimately lead to T cell activation. Fyb protein is located in the cytoplasm and is involved in assembling signaling complexes that facilitate communication between T cells and their environment. Human Fyb gene is located on chromosome 5p13.1 and encodes a 783 amino acid protein, which is essential for maintaining proper immune responses. Anti-Fyb antibody (C-8) enables researchers to gain insights into molecular mechanisms underlying T cell activation and broader implications for immune system function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Fyb Antibody (C-8) References:

1. Molecular alterations of the Fyn-complex occur as late events of human T cell activation.  |  Marie-Cardine, A., et al. 1999. Eur J Immunol. 29: 1175-87. PMID: 10229084
2. SH3 domain recognition of a proline-independent tyrosine-based RKxxYxxY motif in immune cell adaptor SKAP55.  |  Kang, H., et al. 2000. EMBO J. 19: 2889-99. PMID: 10856234
3. Deficiency of ADAP/Fyb/SLAP-130 destabilizes SKAP55 in Jurkat T cells.  |  Huang, Y., et al. 2005. J Biol Chem. 280: 23576-83. PMID: 15849195
4. Molecular basis of the Duffy blood group system.  |  Höher, G., et al. 2018. Blood Transfus. 16: 93-100. PMID: 28151395
5. Anti-Fyb (Duffy) as a cause of transfusion reaction. Case report.  |  Badakere, SS., et al. 1970. Indian J Med Sci. 24: 565-7. PMID: 5484792
6. Fetal development of red cell antigens K, k, Lua, Lub, Fya, Fyb, Vel and Xga.  |  Toivanen, P. and Hirvonen, T. 1969. Scand J Haematol. 6: 49-55. PMID: 5804712
7. Molecular basis and PCR-DNA typing of the Fya/fyb blood group polymorphism.  |  Tournamille, C., et al. 1995. Hum Genet. 95: 407-10. PMID: 7705836
8. Molecular cloning of SKAP55, a novel protein that associates with the protein tyrosine kinase p59fyn in human T-lymphocytes.  |  Marie-Cardine, A., et al. 1997. J Biol Chem. 272: 16077-80. PMID: 9195899
9. Cloning of a novel T-cell protein FYB that binds FYN and SH2-domain-containing leukocyte protein 76 and modulates interleukin 2 production.  |  da Silva, AJ., et al. 1997. Proc Natl Acad Sci U S A. 94: 7493-8. PMID: 9207119
10. Adaptor proteins in lymphocyte antigen-receptor signaling.  |  Peterson, EJ., et al. 1998. Curr Opin Immunol. 10: 337-44. PMID: 9638371
11. FYB (FYN binding protein) serves as a binding partner for lymphoid protein and FYN kinase substrate SKAP55 and a SKAP55-related protein in T cells.  |  Liu, J., et al. 1998. Proc Natl Acad Sci U S A. 95: 8779-84. PMID: 9671755
12. Molecular interaction between the Fyn-associated protein SKAP55 and the SLP-76-associated phosphoprotein SLAP-130.  |  Marie-Cardine, A., et al. 1998. J Biol Chem. 273: 25789-95. PMID: 9748251

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Fyb Antibody (C-8) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Fyb Antibody (C-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Fyb Antibody (C-8) Specifications

Alternate Product Name: Fyb

Product Clone Name: C-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 504-614

Epitope Target: Fyb

Epitope Species: human

Product Reactivity/Detection: Fyb

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Fyb Antibody (C-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin