FUT2 Double Nickase Plasmid (h): sc-409559-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FUT2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FUT2 Double Nickase Plasmid (h) and FUT2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FUT2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FUT2 Antibody (9T-8): sc-100742
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FUT2 Double Nickase Plasmid (h) | sc-409559-NIC | 20 µg | $410.00 | |||
FUT2 Double Nickase Plasmid (h2) | sc-409559-NIC-2 | 20 µg | $410.00 |
Human FUT2 encodes an α(1,2)-fucosyltransferase that catalyzes terminal fucosylation of glycans on secreted and mucosal surface glycoproteins and glycolipids, shaping ABO(H) antigen presentation in secretions. This Golgi-localized enzyme contributes to glycosylation pathways that regulate epithelial barrier interactions, cell–microbe recognition, and lectin-mediated signaling by modifying the glycan landscape. Genetic variation in FUT2 influences secretor status and has been associated with differential susceptibility to enteric infections and with microbiome composition, highlighting its relevance to host–pathogen interfaces. Altered α1,2-fucosylation patterns are also studied in the context of inflammatory disorders and cancer-associated glycan remodeling as functional readouts of glycosyltransferase activity.
FUT2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FUT2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FUT2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FUT2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FUT2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.