FUT2 CRISPR/Cas9 KO Plasmid (h): sc-409559
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FUT2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FUT2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FUT2 Antibody (9T-8): sc-100742
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FUT2 CRISPR/Cas9 KO Plasmid (h) | sc-409559 | 20 µg | $397.00 |
Overview
FUT2 encodes α(1,2)-fucosyltransferase 2, a Golgi-resident glycosyltransferase that catalyzes terminal fucosylation of glycans to generate H type 1/2 antigens on mucosal epithelial surfaces and in secretions. By controlling fucosylated glycan motifs on glycoproteins and glycolipids, FUT2 influences protein trafficking, epithelial barrier properties, and host–microbe interactions at the gut and airway mucosa. Genetic variation and altered FUT2 activity are linked to secretor status and associated differences in mucosal glycosylation patterns that correlate with susceptibility to infectious and inflammatory phenotypes. As a key regulator of glycan-dependent recognition events, FUT2 is widely studied in glycomics, microbiome biology, and epithelial immunology.
FUT2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FUT2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FUT2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FUT2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FUT2 protein expression.
This CRISPR knockout system enables efficient generation of FUT2-deficient cell models for investigation of FUT2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FUT2 exon(s) critical for FUT2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FUT2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FUT2 CRISPR/Cas9 KO Plasmid (h) and FUT2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FUT2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FUT2 HDR Plasmid (h) and FUT2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FUT2 homology arms to support homology-directed repair at defined FUT2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.