Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Furin Double Nickase Plasmid (m): sc-422141-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Furin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Furin Double Nickase Plasmid (m) and Furin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Furin. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Furin Antibody (B-6): sc-133142
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Furin Double Nickase Plasmid (m) | sc-422141-NIC | 20 µg | $410.00 |
Furin (PCSK3) is a calcium-dependent proprotein convertase that processes precursor proteins into mature bioactive forms within the secretory pathway, including the trans-Golgi network and endosomal compartments. By cleaving basic motif substrates, it regulates maturation of hormones, growth factors, receptors, and metalloproteinases, thereby influencing signaling cascades such as TGF-β/BMP, Notch, and extracellular matrix remodeling. In mouse systems, Furin activity shapes developmental patterning, immune and inflammatory responses, and cell–cell communication through controlled proteolysis of membrane and secreted proteins. Dysregulated Furin-dependent processing has been linked in the literature to altered tissue homeostasis and pathophysiology, supporting its use as a mechanistic node in studies of protease-driven signaling and proteostasis.
Furin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Furin locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Furin. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Furin function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Furin-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.