Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
fumarate hydratase Double Nickase Plasmid (h): sc-401660-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- fumarate hydratase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- fumarate hydratase Double Nickase Plasmid (h) and fumarate hydratase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FH. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: fumarate hydratase Antibody (H-6): sc-393992
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
fumarate hydratase Double Nickase Plasmid (h) | sc-401660-NIC | 20 µg | $410.00 | |||
fumarate hydratase Double Nickase Plasmid (h2) | sc-401660-NIC-2 | 20 µg | $410.00 |
Human FH encodes fumarate hydratase (fumarase), a key tricarboxylic acid (TCA) cycle enzyme that catalyzes the reversible hydration of fumarate to L-malate, supporting oxidative metabolism and cellular redox balance. By governing fumarate levels, FH influences mitochondrial bioenergetics and metabolite-dependent signaling, including pathways linked to hypoxia responses and epigenetic regulation. Disruption of FH function is associated with fumarate accumulation and broad metabolic rewiring that can alter oxidative stress handling and transcriptional programs. These properties make FH a widely used node for studying mitochondrial metabolism, oncometabolite biology, and metabolic control of cell state.
fumarate hydratase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FH locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FH. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FH function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FH-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.