FUCA1 Double Nickase Plasmid (h): sc-405275-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FUCA1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FUCA1 Double Nickase Plasmid (h) and FUCA1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FUCA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FUCA1 Antibody (G-12): sc-365496
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FUCA1 Double Nickase Plasmid (h) | sc-405275-NIC | 20 µg | $410.00 | |||
FUCA1 Double Nickase Plasmid (h2) | sc-405275-NIC-2 | 20 µg | $410.00 |
FUCA1 encodes lysosomal alpha-L-fucosidase 1, an exoglycosidase that removes terminal fucose residues from glycoproteins and glycolipids during glycan turnover. By supporting lysosome-dependent catabolism and quality control of fucosylated substrates, FUCA1 influences cellular homeostasis, endo-lysosomal trafficking, and the composition of cell-surface and secreted glycoconjugates. Altered FUCA1 activity is linked to disrupted lysosomal function and aberrant glycosylation patterns that can affect adhesion, receptor signaling, and immune-related processes. In biomedical research, FUCA1 is commonly interrogated in studies of lysosomal storage biology, glycoprotein degradation pathways, and disease-associated changes in fucosylation.
FUCA1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FUCA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FUCA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FUCA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FUCA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.