FTα Double Nickase Plasmid (h): sc-403920-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FTα Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FTα Double Nickase Plasmid (h) and FTα Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FNTA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FTα Antibody (D-5): sc-374262
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FTα Double Nickase Plasmid (h) | sc-403920-NIC | 20 µg | $410.00 | |||
FTα Double Nickase Plasmid (h2) | sc-403920-NIC-2 | 20 µg | $410.00 |
FNTA encodes the alpha subunit of farnesyltransferase (FTα), which forms an obligate heterodimer with the beta subunit to catalyze protein farnesylation, a C15 isoprenoid lipid modification on C-terminal CAAX motifs. This post-translational processing supports membrane association and subcellular trafficking of diverse signaling proteins, including members of the RAS superfamily, thereby influencing MAPK signaling, cytoskeletal organization, vesicle dynamics, and cell-cycle progression. FTα activity links the mevalonate/isoprenoid biosynthesis pathway to proteostasis and signal transduction by enabling stable localization and function of prenylated substrates. Dysregulated prenylation and altered FNTA-dependent processing have been associated with aberrant growth signaling and oncogenic pathways, making FNTA a useful target for dissecting prenylation-dependent network behavior in human cells.
FTα Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FNTA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FNTA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FNTA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FNTA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.