FSD1 CRISPR/Cas9 KO Plasmid (h): sc-406328
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FSD1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FSD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FSD1 CRISPR/Cas9 KO Plasmid (h) | sc-406328 | 20 µg | $397.00 |
Overview
FSD1 (fibronectin type III and SPRY domain containing 1) encodes a cytosolic protein that contains SPRY and fibronectin type III–like domains, suggesting a role in protein–protein interactions and regulation of signaling complexes. Reported functional links place FSD1 in the control of cytoskeletal organization and microtubule-associated processes, with potential impacts on cellular architecture, trafficking, and stress responses. Expression and genomic alterations involving FSD1 have been noted in cancer datasets, supporting its use as a candidate gene for studying mechanisms that influence proliferation, migration, and cellular fitness. As a relatively under-characterized factor, FSD1 is also relevant for pathway discovery studies that connect domain-mediated scaffolding proteins to downstream signaling outputs.
FSD1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FSD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FSD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FSD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FSD1 protein expression.
This CRISPR knockout system enables efficient generation of FSD1-deficient cell models for investigation of FSD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FSD1 exon(s) critical for FSD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FSD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FSD1 CRISPR/Cas9 KO Plasmid (h) and FSD1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FSD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FSD1 HDR Plasmid (h) and FSD1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FSD1 homology arms to support homology-directed repair at defined FSD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.