fractalkine CRISPR Activation Plasmid (h): sc-402339-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- fractalkine CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- fractalkine CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by fractalkine CRISPR Activation Plasmid (h) and fractalkine CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the CX3CL1 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: fractalkine Antibody (A-9): sc-166200
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
fractalkine CRISPR Activation Plasmid (h) | sc-402339-ACT | 20 µg | $397.00 |
CX3CL1 encodes fractalkine, a unique CX3C chemokine that exists as both a membrane-tethered adhesion molecule and a soluble chemoattractant. Through its receptor CX3CR1, fractalkine regulates leukocyte adhesion, chemotaxis, and microglia–neuron communication, linking chemokine signaling to inflammatory trafficking and tissue surveillance. CX3CL1–CX3CR1 activity intersects with NF-κB-driven inflammatory programs and modulates endothelial activation and immune cell recruitment. Dysregulated fractalkine signaling has been associated with neuroinflammation, vascular inflammation, and tumor–immune interactions, making CX3CL1 a useful node for studying immune–stromal crosstalk and inflammatory pathobiology.
fractalkine CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous CX3CL1 expression without altering the underlying DNA sequence.
fractalkine CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the CX3CL1 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the CX3CL1 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous fractalkine expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native CX3CL1 locus and enabling the study of fractalkine-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of fractalkine pathway restoration in tumor cells with silenced or reduced CX3CL1 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.