Fra2 Double Nickase Plasmid (h): sc-400250-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Fra2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Fra2 Double Nickase Plasmid (h) and Fra2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FOSL2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Fra2 Antibody (G-5): sc-166102
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fra2 Double Nickase Plasmid (h) | sc-400250-NIC | 20 µg | $410.00 | |||
Fra2 Double Nickase Plasmid (h2) | sc-400250-NIC-2 | 20 µg | $410.00 |
Human FOSL2 encodes Fra2, an AP-1 family bZIP transcription factor that heterodimerizes with JUN proteins to regulate stimulus-responsive gene expression. Fra2 integrates MAPK/ERK and JNK signaling to control proliferation, differentiation, extracellular matrix remodeling, and inflammatory transcriptional programs. Through AP-1–dependent regulation of cytokines, metalloproteinases, and EMT-associated genes, altered FOSL2 activity has been linked to oncogenic signaling, fibrosis-related pathways, and immune dysregulation in multiple tissue contexts. Fra2 is therefore widely used as a molecular node for studying transcriptional network rewiring downstream of growth factors, stress cues, and developmental signals.
Fra2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FOSL2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FOSL2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FOSL2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FOSL2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.