Fra2 Antibody (G-5): sc-166102

Fra-2 Antibody (G-5) is a mouse monoclonal IgG2a kappa light chain antibody raised against amino acids 180-282 of human Fra-2 (Fos-related antigen 2/FOSL2), a crucial nuclear transcription factor. Anti-Fra-2 antibody (G-5) recognizes an epitope within this region and demonstrates broad species cross-reactivity, detecting Fra-2 in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications. Fra-2 maintains precise nuclear localization, where Fra-2 forms heterodimers with Jun family proteins, particularly c-Jun, through specialized leucine zipper and basic regions. These structural elements prove essential for both dimerization and DNA binding, enabling Fra-2-Jun complexes to recognize specific DNA sequences called TPA-responsive elements (TRE) within target gene promoters. Through this sophisticated mechanism, Fra-2 modulates transcriptional activity within the Activator Protein-1 (AP-1) complex, orchestrating the expression of genes controlling cell proliferation, differentiation, and apoptosis. Through these diverse cellular functions, Fra-2 (G-5) antibody provides researchers with a valuable tool for investigating transcriptional regulation and fundamental cellular processes.

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Fra2 Antibody (G-5) References:

1. The transcriptional response to hypoxic insult controlled by FRA-2.  |  Butler, TL. and Pennypacker, KR. 2005. Gene Expr. 12: 61-7. PMID: 15892448
2. Inhibition of NFAT increases osteoblast differentiation by increasing Fra-2 expression.  |  Zayzafoon, M. 2005. J Musculoskelet Neuronal Interact. 5: 347. PMID: 16340132
3. Identification of FRA1 and FRA2 as genes involved in regulating the yeast iron regulon in response to decreased mitochondrial iron-sulfur cluster synthesis.  |  Kumánovics, A., et al. 2008. J Biol Chem. 283: 10276-86. PMID: 18281282
4. Isolation of human fos-related genes and their expression during monocyte-macrophage differentiation.  |  Matsui, M., et al. 1990. Oncogene. 5: 249-55. PMID: 2107490
5. Isolation and characterization of fra-2, an additional member of the fos gene family.  |  Nishina, H., et al. 1990. Proc Natl Acad Sci U S A. 87: 3619-23. PMID: 2110368
6. Fra2 is a co-regulator of Fep1 inhibition in response to iron starvation.  |  Jacques, JF., et al. 2014. PLoS One. 9: e98959. PMID: 24897379
7. The product of a fos-related gene, fra-1, binds cooperatively to the AP-1 site with Jun: transcription factor AP-1 is comprised of multiple protein complexes.  |  Cohen, DR., et al. 1989. Genes Dev. 3: 173-84. PMID: 2497053
8. The product of a novel growth factor activated gene, fos B, interacts with JUN proteins enhancing their DNA binding activity.  |  Zerial, M., et al. 1989. EMBO J. 8: 805-13. PMID: 2498083
9. Fos-associated protein p39 is the product of the jun proto-oncogene.  |  Rauscher, FJ., et al. 1988. Science. 240: 1010-6. PMID: 3130660
10. The Fos protein complex is associated with DNA in isolated nuclei and binds to DNA cellulose.  |  Sambucetti, LC. and Curran, T. 1986. Science. 234: 1417-9. PMID: 3491427
11. ATRA ameliorates fibrosis by suppressing the pro-fibrotic molecule Fra2/AP-1 in systemic sclerosis.  |  Pi, Z., et al. 2023. Int Immunopharmacol. 121: 110420. PMID: 37331293
12. Viral and cellular fos proteins are complexed with a 39,000-dalton cellular protein.  |  Curran, T., et al. 1985. Mol Cell Biol. 5: 167-72. PMID: 3885008

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Fra2 Antibody (G-5) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Fra2 Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Fra2 Antibody (G-5) Specifications

Alternate Product Name: Fra-2

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 180-282

Epitope Target: Fra-2

Epitope Species: human

Product Reactivity/Detection: Fra-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Fra2 Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Fos B antibody is Fos B Antibody (F-7): sc-398595.