FR Antibody (E-11): sc-515521

FR Antibody (E-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects folate receptor (FR) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). FR Antibody (E-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. The folate receptor family plays a crucial role in cellular uptake of folate, an essential vitamin necessary for DNA synthesis and repair, as well as amino acid metabolism. This family consists of five members: α, β, γ, γ′ (produced by alternative splicing), and δ, with α-FR and β-FR being particularly significant as they are expressed in malignant tissues, making them valuable targets for cancer therapy. The unique structure of these receptors, which includes a glycosylphosphatidylinositol (GPI) anchor for α-FR and β-FR, facilitates their localization to the cell membrane, enhancing their ability to mediate the endocytosis of 5-methyltetrahydrofolate. This mechanism is vital for maintaining folate homeostasis in cells, especially in rapidly dividing tissues, and underscores the importance of folate receptors in both normal physiology and cancer biology. α-FR serves as a highly selective tumor marker, offering potential for targeted drug delivery systems that utilize folate derivatives to selectively transport therapeutic agents to tumor cells, thereby improving treatment efficacy while minimizing systemic toxicity.

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FR Antibody (E-11) References:

1. Targeted drug delivery via the folate receptor.  |  Sudimack, J. and Lee, RJ. 2000. Adv Drug Deliv Rev. 41: 147-62. PMID: 10699311
2. Differentiation-independent retinoid induction of folate receptor type beta, a potential tumor target in myeloid leukemia.  |  Wang, H., et al. 2000. Blood. 96: 3529-36. PMID: 11071651
3. Adaptive regulation of intestinal folate uptake: effect of dietary folate deficiency.  |  Said, HM., et al. 2000. Am J Physiol Cell Physiol. 279: C1889-95. PMID: 11078704
4. Identification of two putative novel folate receptor genes in humans and mouse.  |  Spiegelstein, O., et al. 2000. Gene. 258: 117-25. PMID: 11111049
5. The rate of folate receptor alpha (FR alpha) synthesis in folate depleted CHL cells is regulated by a translational mechanism sensitive to media folate levels, while stable overexpression of its mRNA is mediated by gene amplification and an increase in transcript half-life.  |  Zhu, WY., et al. 2001. J Cell Biochem. 81: 205-19. PMID: 11241661
6. Targeting the folate receptor (FR): imaging and cytotoxicity of ReI conjugates in FR-overexpressing cancer cells.  |  Viola-Villegas, N., et al. 2008. ChemMedChem. 3: 1387-94. PMID: 18651631
7. Development of a folate receptor (FR)-targeted indenoisoquinoline using a pH-sensitive N-ethoxybenzylimidazole (NEBI) bifunctional cross-linker.  |  Cao, Y. and Yang, J. 2014. Bioconjug Chem. 25: 873-8. PMID: 24758386
8. Synthesis and preliminary evaluation of a 99m Tc-labeled folate-PAMAM dendrimer for FR imaging.  |  Song, M., et al. 2017. Chem Biol Drug Des. 89: 755-761. PMID: 27910223
9. Clinical significance of postoperative folate receptor-positive circulating tumor cells (FR + CTCs) for long-term prognosis in patients with invasive adenocarcinoma (IAC) of the lung.  |  Ma, Z., et al. 2024. Thorac Cancer. 15: 1060-1071. PMID: 38532562
10. Folate receptor type gamma is primarily a secretory protein due to lack of an efficient signal for glycosylphosphatidylinositol modification: protein characterization and cell type specificity.  |  Shen, F., et al. 1995. Biochemistry. 34: 5660-5. PMID: 7727426
11. Selective expression of the high-affinity isoform of the folate receptor (FR-alpha) in the human placental syncytiotrophoblast and choriocarcinoma cells.  |  Prasad, PD., et al. 1994. Biochim Biophys Acta. 1223: 71-5. PMID: 8061055
12. Structure and regulation of a polymorphic gene encoding folate receptor type gamma/gamma'.  |  Wang, H., et al. 1998. Nucleic Acids Res. 26: 2132-42. PMID: 9547271

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FR Antibody (E-11) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-FR Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FR Antibody (E-11) Specifications

Alternate Product Name: FR

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 210-231

Epitope Target: FR

Epitope Species: human

Product Reactivity/Detection: α-FR, β-FR and γ-FR of human origin and α-FR, and β-FR of mouse and rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for FR Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin