FOXP2 Double Nickase Plasmid (h): sc-417494-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FOXP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FOXP2 Double Nickase Plasmid (h) and FOXP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FOXP2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FOXP2 Antibody (5C11A8): sc-517261
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOXP2 Double Nickase Plasmid (h) | sc-417494-NIC | 20 µg | $410.00 |
FOXP2 encodes a forkhead box transcription factor that regulates gene expression programs required for neurodevelopment, neuronal differentiation, and circuit formation. In the nucleus, FOXP2 binds specific DNA motifs and coordinates transcriptional networks involved in synaptic plasticity, neurite outgrowth, and activity-dependent signaling, interfacing with pathways that shape cortical and basal ganglia development. Altered FOXP2 dosage or sequence is linked to speech and language impairment and broader neurodevelopmental phenotypes, making it a key node for studying transcriptional control of communication-related neural circuits. FOXP2 is also investigated in models of neuronal maturation and cell-type specification to map downstream targets and regulatory architecture.
FOXP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FOXP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FOXP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FOXP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FOXP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.