FOXP1 Double Nickase Plasmid (h): sc-418144-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FOXP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FOXP1 Double Nickase Plasmid (h) and FOXP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FOXP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FOXP1 Antibody (A-2): sc-398811
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOXP1 Double Nickase Plasmid (h) | sc-418144-NIC | 20 µg | $410.00 |
FOXP1 encodes a forkhead box transcription factor that regulates lineage specification, cell-cycle control, and differentiation programs through sequence-specific DNA binding and transcriptional repression or activation. In immune and hematopoietic systems, FOXP1 influences B cell maturation, T cell quiescence, and transcriptional networks downstream of cytokine and antigen receptor signaling, while in the nervous system it contributes to neurodevelopmental patterning and synaptic gene expression. FOXP1 activity intersects with chromatin remodeling complexes and developmental pathways that coordinate proliferation and fate decisions. Dysregulated FOXP1 expression or mutation has been associated with neurodevelopmental phenotypes and with altered transcriptional states observed across multiple tumor contexts, supporting its use as a mechanistic node in transcriptional circuit studies.
FOXP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FOXP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FOXP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FOXP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FOXP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.