Anti-FOXN4 Antibody (G-1) is a mouse monoclonal IgG1 (kappa light chain) FOXN4 antibody provided at 200 µg/ml
raised against amino acids 1-195 mapping at the N-terminus of FOXN4 of human origin
Anti-FOXN4 Antibody (G-1) is recommended for detection of FOXN4 of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-FOXN4 Antibody (G-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of FOXN4 (G-1): sc-377166.
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Labware ships FedEx Ground free of charge to the contiguous US
FOXN4 Antibody (G-1) is a high quality monoclonal FOXN4 antibody (also designated FOXN4 antibody) suitable for the detection of the FOXN4 protein of mouse, rat and human origin. FOXN4 Antibody (G-1) is available as both the non-conjugated anti-FOXN4 antibody form, as well as multiple conjugated forms of anti-FOXN4 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The human gene FOXN4 encodes for a 476 amino acid nuclear protein designated FOXN4. FOXN4 cooperates with key retinogenic factors to mediate the multipotent differentiation of retinal progenitors and is believed to regulate neuronal subtype diversification. FOXN4 is expressed in a subset of mitotic progenitors during retinogenesis. As such, FOXN4 controls the formation of amacrine and horizontal cells by activating the expression of the retinogenic factors MATH-3, Neuro D and PROX1. During spinal neurogenesis, the p2 progenitor domain gives rise to two intermingled distinct subtypes of interneurons, termed V2a and V2b. FOXN4 is coexpressed with the bHLH factor ASH1 (Mash1) in a subset of p2 progenitors. Functionality of FOXN4 affects ASH1 expression and regulates interneuronal formation accordingly. Over-expression of FOXN4 alone in spinal neural progenitors promotes the V2a fate at the expense of the V2b fate, whereas ASH1 suppresses both the V2a and V2b fates.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended starting dilution for Western blot for sc-377166: FOXN4 (G-1) monoclonal antibody?
Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: