FOXL2 Double Nickase Plasmid (h): sc-403351-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FOXL2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FOXL2 Double Nickase Plasmid (h) and FOXL2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FOXL2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FOXL2 Antibody (262C1a): sc-81275
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOXL2 Double Nickase Plasmid (h) | sc-403351-NIC | 20 µg | $410.00 | |||
FOXL2 Double Nickase Plasmid (h2) | sc-403351-NIC-2 | 20 µg | $410.00 |
FOXL2 encodes a forkhead family transcription factor that is essential for ovarian granulosa cell differentiation, maintenance of ovarian identity, and regulation of genes involved in steroidogenesis and folliculogenesis. Through sequence-specific DNA binding, FOXL2 integrates developmental signaling with transcriptional programs controlling cell fate decisions, apoptosis, and reproductive endocrine homeostasis. It participates in gene regulatory networks that intersect with TGF-β/SMAD signaling and other pathways governing gonadal development. Dysregulation or mutation of FOXL2 is linked to disorders of sex development and ovarian pathologies, including characteristic alterations observed in granulosa cell tumors.
FOXL2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FOXL2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FOXL2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FOXL2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FOXL2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.