FOXJ1 Lentiviral Activation Particles (h): sc-402226-LAC

Human FOXJ1 encodes a forkhead box transcription factor that serves as a master regulator of motile ciliogenesis and epithelial cell differentiation. FOXJ1 coordinates transcriptional programs controlling basal body docking, axonemal assembly, and dynein arm expression, integrating with developmental signaling networks such as Notch and Wnt to establish ciliated cell fate. Through its role in airway and ependymal cilia formation, altered FOXJ1 activity is linked to impaired mucociliary clearance and dysregulated cerebrospinal fluid flow, processes relevant to primary ciliary dyskinesia–like phenotypes and other ciliopathies. FOXJ1 is also used as a marker and functional node for studying multiciliated epithelia, lineage specification, and transcriptional control of ciliary gene networks.

FOXJ1 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient FOXJ1 upregulation across a broader range of human cell types.

FOXJ1 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the FOXJ1 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous FOXJ1 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native FOXJ1 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.