FOXF2 Double Nickase Plasmid (h): sc-404367-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FOXF2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FOXF2 Double Nickase Plasmid (h) and FOXF2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FOXF2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FOXF2 Antibody (L-29): sc-101043
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOXF2 Double Nickase Plasmid (h) | sc-404367-NIC | 20 µg | $410.00 | |||
FOXF2 Double Nickase Plasmid (h2) | sc-404367-NIC-2 | 20 µg | $410.00 |
FOXF2 (forkhead box F2) encodes a Forkhead family transcription factor that regulates mesenchymal differentiation programs and epithelial–mesenchymal signaling during tissue development and homeostasis. FOXF2 modulates gene networks linked to extracellular matrix organization, cell migration, and angiogenic remodeling, with functional crosstalk to pathways such as TGF-β/SMAD and Wnt/β-catenin that shape stromal and vascular phenotypes. Altered FOXF2 expression or regulatory activity has been associated with dysregulated developmental patterning and context-dependent roles in tumor biology, including effects on invasion, metastasis-associated stroma, and vascular microenvironment. As a nuclear DNA-binding protein, FOXF2 is frequently studied to map lineage-specific transcriptional circuitry and to dissect gene–environment interactions that influence tissue architecture.
FOXF2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FOXF2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FOXF2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FOXF2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FOXF2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.